Isolation and Characterization of Cellulase Producing Streptomyces albaduncus from Gut of Eisenia foetida
نویسندگان
چکیده
Cellulose is the most abundant biopolymer on earth, recognized as the major component of plant biomass. The cellulolytic microorganisms were isolated from gut of earthworm (Eisenia foetida). Using Glycerol asparagine agar media, the organisms were identified as Actinomycetes sps.. The endoglucanase activity of the culture broth was determined during actinomycetes growth by measuring the release of reducing sugar from carboxyl methyl cellulose, mineral salts medium. The reducing sugar was assayed by the dinitro salicylic acid method, using glucose ass the sugar standard. One unit of cellulose was defined as the amount of enzyme which produced one micro mole glucose equivalent per minute under the assay condition. The production of enzyme in Mineral salts medium was studied for the effect of temperature and pH. The production of cellulose enzyme was low and at 37C to 50C optimum was observed. The optimum production of cellulose was observed at pH of 6.5-7.0.
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